Determination of selected xenobiotics with ferrofluid-modified trypsin

Authors: Scaronafarcaroník I.1; Ptáccaronková L.2; Koneracká M.3; Scaronafarcaroníková M.2; Timko M.3; Kopccaronanský P.3

Source: Biotechnology Letters, Volume 24, Number 5, March 2002 , pp. 355-358(4)

Publisher: Springer

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Abstract:

Ferrofluid-modified trypsin has been used for the detection and determination of selected xenobiotics that inhibit trypsin activity. The procedure is useful especially when colored samples or samples containing suspended solid impurities are to be assayed. Ferrofluid-modified trypsin was inhibited by Ag^+ and Pb^2+, selected dyes (safranin, thionin), bacitracin and 4-aminobenzamidine. Enzymes immobilized on magnetic particles can form a basis of new automated assay procedures for the determination of xenobiotics.

Keywords: ferrofluid-modified trypsin; magnetic fluids; trypsin inhibitors

Language: English

Document Type: Regular paper

Affiliations: 1: E-mail: safarik@uek.cas.cz) 2: Laboratory of Biochemistry and Microbiology, Institute of Landscape Ecology, Na Sádkách 7, 370 05 Ccaroneské Budecaronjovice, Czech Republic eské Budecaronjovice, Czech Republic "> 3: Department of Magnetism, Institute of Experimental Physics, Watsonova 47, 04353 Koscaronice, Slovak Republicice, Slovak Republic">

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