Bioreactor production of camptothecin by hairy root cultures of Ophiorrhiza pumila

Authors: Sudo H.¹; Yamakawa T.²; Yamazaki M.¹; Aimi N.³; Saito K.⁴

Source: Biotechnology Letters, Volume 24, Number 5, March 2002 , pp. 359-363(5)

Publisher: Springer

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Abstract:

A large-scale culture of hairy root of Ophiorrhiza pumila using a modified 3 l bioreactor was established. The hairy roots, incited by infection of Agrobacterium rhizogenes were grown in the bioreactor equipped with a stainless net. The final concentration of camptothecin was 0.0085% fresh wt of tissue, and the total production of camptothecin, an anti-neoplastic quinoline alkaloid, reached 22 mg over 8 weeks' culture in the reactor. Approx. 17% (3.6 mg) of the total camptothecin produced was excreted into the culture medium.

Keywords: bioreactor; camptothecin; excretion; hairy root; Ophiorrhiza pumila

Language: English

Document Type: Regular paper

Affiliations: 1: Department of Molecular Biology and Biotechnology and Department of Molecular Structure and Biological Function, Graduate school of Pharmaceutical Sciences, Chiba University, Yayoi-cho 1-33, Inage-ku, Chiba 263-8522, Japan 2: Department of Global Agricultural Sciences, The University of Tokyo, Yayoi 1-chome, Bunkyo-ku, Tokyo 113-8657, Japan 3: Department of Molecular Structure and Biological Function, Graduate school of Pharmaceutical Sciences, Chiba University, Yayoi-cho 1-33, Inage-ku, Chiba 263-8522, Japan 4: E-mail: ksaito@p.chiba-u.ac.jp)

Publication date: 2002-03-01