Leaf developmental stage and tissue location affect the detection of bgr-glucuronidase in transgenic tobacco plants

Authors: Pret'ová A.1; Obert B.1; Wetzstein H.Y.2

Source: Biotechnology Letters, Volume 23, Number 7, April 2001 , pp. 555-558(4)

Publisher: Springer

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Abstract:

Expression in Nicotiana tabaccum L. plants containing the bgr-glucuronidase (GUS) gene under the control of the 35S (CaMV promoter) was affected by tissue type and ontogenic development of the leaves. GUS activity in ontogenetically younger leaves was 1003–1022 nmol 7-hydroxy-4-methylcoumarin (MU) formed mg^−1 (protein) min^−1 and in ontogenetically older leaves was only 140–198 nmol (MU) mg^−1 (protein) min^−1.

Keywords: bgr-glucuronidase; genetic transformation; Nicotiana tabaccum L. cv. Petite Havana

Language: English

Document Type: Regular paper

Affiliations: 1: Institute of Plant Genetics and Biotechnology. Slovak Academy of Sciences, Akademická 2, P.O. Box 39/A, 95007 Nitra, Slovak Republic 2: Horticulture Department, 1111 Plant Science Building, University of Georgia, Athens, GA 30602-7273, USA

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