Improved ethanol tolerance of Saccharomyces cerevisiae strains by increases in fatty acid unsaturation via metabolic engineering

Authors: Kajiwara S.¹; Suga K.²; Sone H.³; Nakamura K.³

Source: Biotechnology Letters, Volume 22, Number 23, December 2000 , pp. 1839-1843(5)

Publisher: Springer

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Abstract:

To enhance the ethanol tolerance of Saccharomyces cerevisiae, the Arabidopsis thaliana FAD2 gene and/or the S. cerevisiae OLE1 gene were over-expressed in this yeast. The transformant over-expressing both these genes could not only synthesize dienoic fatty acids but also increased the unsaturated fatty acid content of membrane lipid and then showed the highest viability in the presence of 15% (v/v) ethanol.

Keywords: desaturase; ethanol tolerance; fatty acid; yeast

Language: English

Document Type: Regular paper

Affiliations: 1: Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226-8501, Japan 2: Department of Chemical Engineering, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8552, Japan 3: Central Laboratories for Key Technology, Kirin Brewery Co., Ltd. 1-13-5 Fukuura Kanazawa-ku, Yokohama 236-0004, Japan

Publication date: 2000-12-01