Optical resolution of RS-(±)-mandelic acid by Pseudomonas sp.

Authors: Kim B-Y.¹; Hwang K-C.¹; Song H-S.¹; Chung N.¹; Bang W-G.¹

Source: Biotechnology Letters, Volume 22, Number 23, December 2000 , pp. 1871-1875(5)

Publisher: Springer

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Abstract:

For the optical resolution of R-(-)-mandelic acid from (±)-mandelic acid, Pseudomonas sp. MA02, which assimilated S-(+)-mandelic acid as carbon and energy source, was isolated from soil. Using the fed-batch culture under optimal condition, R-(-)-mandelic acid was accumulated up to the maximum theoretical yield of 50% (30 g l^−1) and entiomeric excess of 99.4%.

Keywords: fed-batch fermentation; mandelic acid; optical resolution; Pseudomonas sp.

Language: English

Document Type: Regular paper

Affiliations: 1: E-mail: agrchem@mail.korea.ac.kr)

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