Scopolamine and hyoscyamine production by hairy root cultures of Brugmansia candida: influence of calcium chloride, hemicellulase and theophylline

Authors: Pitta-Alvarez S.I.; Spollansky T.C.; Giulietti A.M.

Source: Biotechnology Letters, Volume 22, Number 20, October 2000 , pp. 1653-1656(4)

Publisher: Springer

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Abstract:

CaCl_2 (50 mM) and hemicellulase (0.5 U mg^−1) increased the intracellular accumulation (60–250%), release (60–200%) and production (45–200%) of hyoscyamine and scopolamine in hairy roots of Brugmansia candida. Theophylline (0.25 mM), alone or in combination with hemicellulase, was ineffective in increasing hyoscyamine and scopolamine production.

Keywords: Brugmansia candida; hairy root cultures; hyoscyamine; scopolamine

Language: English

Document Type: Regular paper

Affiliations: 1: E-mail: agiule@ffyb.uba.ar)

Publication date: 2000-10-01