@article {Karim:October 2000:0141-5492:1647, author = "Karim N.", author = "Allmeling C.", author = "Hengstler J-G.", author = "Haverich A.", author = "Bader A.", title = "Diazepam metabolism and albumin secretion of porcine hepatocytes in collagen-sandwich after cryopreservation", journal = "Biotechnology Letters", volume = "22", year = "October 2000", abstract = "

Cryopreserved porcine hepatocytes in collagen cultures secreted albumin (up to 98 ± 3 mgrg ml^−1; non-cryopreserved controls: 100 ± 9 mgrg ml^−1) and metabolised diazepam (67.5% ± 7.5% of initially applied diazepam are metabolised; 77.5% ± 10% in non-cryopreserved controls) for up to 14 days after thawing. The cultures resembled the recently developed flat membrane bioreactors. Addition of 5% (v/v) serum did not effect diazepam metabolism. Hepatocytes in collagen-sandwich cultures offer a storable liver support system for potential clinical use.

", pages = "1647-1652(6)", url = "http://www.ingentaconnect.com/content/klu/bile/2000/00000022/00000020/00281180" }