Diazepam metabolism and albumin secretion of porcine hepatocytes in collagen-sandwich after cryopreservation

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In this Subject: Biology ,&nbsp; Biotechnology ,&nbsp; Microbiology
By this author: Karim N. ;&nbsp; Allmeling C. ;&nbsp; Hengstler J-G. ;&nbsp; Haverich A. ;&nbsp; Bader A.

Authors: Karim N.¹; Allmeling C.¹; Hengstler J-G.²; Haverich A.¹; Bader A.¹

Source: Biotechnology Letters, Volume 22, Number 20, October 2000 , pp. 1647-1652(6)

Publisher: Springer

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Abstract:

Cryopreserved porcine hepatocytes in collagen cultures secreted albumin (up to 98 ± 3 mgr g ml^−1; non-cryopreserved controls: 100 ± 9 mgr g ml^−1) and metabolised diazepam (67.5% ± 7.5% of initially applied diazepam are metabolised; 77.5% ± 10% in non-cryopreserved controls) for up to 14 days after thawing. The cultures resembled the recently developed flat membrane bioreactors. Addition of 5% (v/v) serum did not effect diazepam metabolism. Hepatocytes in collagen-sandwich cultures offer a storable liver support system for potential clinical use.