Diazepam metabolism and albumin secretion of porcine hepatocytes in collagen-sandwich after cryopreservation

Authors: Karim N.1; Allmeling C.1; Hengstler J-G.2; Haverich A.1; Bader A.1

Source: Biotechnology Letters, Volume 22, Number 20, October 2000 , pp. 1647-1652(6)

Publisher: Springer

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Abstract:

Cryopreserved porcine hepatocytes in collagen cultures secreted albumin (up to 98 ± 3 mgrg ml^−1; non-cryopreserved controls: 100 ± 9 mgrg ml^−1) and metabolised diazepam (67.5% ± 7.5% of initially applied diazepam are metabolised; 77.5% ± 10% in non-cryopreserved controls) for up to 14 days after thawing. The cultures resembled the recently developed flat membrane bioreactors. Addition of 5% (v/v) serum did not effect diazepam metabolism. Hepatocytes in collagen-sandwich cultures offer a storable liver support system for potential clinical use.

Keywords: artificial liver; cryopreservation; hepatocyte metabolism

Language: English

Document Type: Regular paper

Affiliations: 1: Leibniz Research Laboratories 2: Institute of Toxicology, Gutenberg-University, Mainz, Germany

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