Identification, Inheritance, and Variation of Microsatellite Markers in the Black Scallop Mimachlamys varia
Source: Biochemical Genetics, Volume 49, Numbers 3-4, April 2011 , pp. 139-152(14)
Abstract:Five polymorphic microsatellite loci were identified in the black scallop Mimachlamys varia after construction of a genomic library enriched for (GT)n. To examine the transmission pattern of microsatellite alleles, several families were created and genotypes scored for three loci. The expected Mendelian ratios were found in 12 of 14 segregations examined. Unexpected segregations may be explained by a genotyping error (allelic dropout), given that when a specific allele was treated as dominant, the phenotypic ratios conformed to Mendelian expectations. The five loci were also examined in two samples from the Spanish coast. The two localities displayed similar mean values for the number of alleles per locus (7.2–8.4), allelic richness (7.2–7.9), and observed (0.389–0.484) and expected heterozygosity (0.545–0.618). Significant Hardy–Weinberg deviations were observed at three loci, with heterozygote deficiency occurring in all cases. Global multilocus value and allele frequencies at one locus revealed significant differentiation between the two localities.
Document Type: Research Article
Affiliations: 1: Departamento de Biología Celular y Molecular, Universidade da Coruña, A Zapateira s/n, 15071, A Coruña, Spain 2: Instituto Español de Oceanografía, Centro Oceanográfico de A Coruña, A Coruña, Spain 3: Departamento de Biología Celular y Molecular, Universidade da Coruña, A Zapateira s/n, 15071, A Coruña, Spain, Email: firstname.lastname@example.org
Publication date: 2011-04-01