Inorganic Pyrophosphatase: A New Polymorphic Allozyme Locus in Pacific Salmon

Authors: Kuligowski D.R.¹; Winans G.A.²

Source: Biochemical Genetics, Volume 40, Numbers 11-12, December 2002 , pp. 379-385(7)

Publisher: Springer

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Abstract:

Genetic polymorphism of inorganic pyrophosphatase was investigated in 2799 individuals in four species of Pacific salmon: chinook salmon (Oncorhynchus tshawytscha), coho salmon (O. kisutch), kokanee (O. nerka), and steelhead(O. mykiss), using horizontal starch gel electrophoresis. This enzyme system appears to be an isolocus system with electrophoretically indistinguishable allozymes encoded by two loci (PP-1,2*) expressed in retinal tissue. Mendelian inheritance was observed with a breeding study in three family crosses. Population variability in four species was characterized in 44 populations from the U.S. Pacific coast. Three alleles were found in chinook salmon; two alleles each were found in coho salmon, kokanee, and steelhead. Chinook salmon and kokanee populations differed enough with respect to PP-1,2* frequencies that this isolocus is useful for genetic stock identification in these species.

Keywords: inorganic pyrophosphatase; genetic polymorphism; isolocus; electrophoresis; Pacific salmon

Language: English

Document Type: Research article

Affiliations: 1: National Marine Fisheries Service, Manchester Research Station, Post Office Box 130, Manchester, Washington 98353; david.kuligowski@noaa.gov 2: Conservation Biology Division, Northwest Fisheries Science Center, 2725 Montlake Boulevard East, Seattle, Washington 98112-2097

Publication date: 2002-12-01