Potassium Assay in Blastomere of Two-Cell Mouse Embryo after Equilibration and washing from Cryoprotector

Authors: D. V. Goldshtein; E. I. Smolyaninova; A. G. Pogorelov

Source: Bulletin of Experimental Biology and Medicine, Volume 138, Number 1, July 2004 , pp. 40-41(2)

Publisher: Springer

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Abstract:

The need for effective methods of cryopreservation of early mammalian embryos necessitates the study of the mechanisms of blastomere adaptation to the equilibration procedure and subsequent washing from the cryoprotector. The osmotic effects during these procedures can cause electrolyte imbalance in embryonic cells. Intracellular potassium concentrations at the stage of two-blastomere mouse embryo were studied by electron probe microanalysis.

Keywords: cryopreservation; ethylene glycol; two-blastomere embryo; cytoplasmatic potassium; electron probe microanalysis

Document Type: Research article

DOI: http://dx.doi.org/10.1023/B:BEBM.0000046933.90918.1d

Affiliations: 1: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino. , pogorelov@iteb.ru, Email: pogorelov_a@mail.ru, pogorelov@iteb.ru

Publication date: 2004-07-01