Authors: Caille, Nicolas; Rodina, Marek; Kocour, Martin; Gela, David; Flajšhans, Martin; Linhart, Otomar

Source: Aquaculture International, Volume 14, Numbers 1-2, February 2006 , pp. 75-87(13)

Publisher: Springer

Abstract:

The spermiation of tench males was stimulated with Supergestran containing mammalian LHRHa lecireline at the following doses: 5, 10, 20 and 40 <EquationSource Format="TEX"><![CDATA[$$upmu$$]]></EquationSource> g kg⁻¹ b.w.; then with carp pituitary suspension (CPS) at a dose of 2 mg kg⁻¹ b.w. and with a control of saline physiological solution. The following days, meaning 24, 48 and 72 h after injection, sperm was collected to evaluate volume and the number of sperm per male per kg body weight (B.W.) The percentage of motile sperm and velocity of spermatozoa were measured 48 h after hormonal injection, and 72 h after hormonal injection the sperm was evaluated for fertilization and hatching ability. All 42 males in experimental groups were diploid. Live weight did not differ significantly among experimental groups. The strongest stimulation of spermiation was achieved with LHRHa in dosage of 20 and 40 <EquationSource Format="TEX"><![CDATA[$$upmu$$]]></EquationSource> g kg⁻¹ b.w. and CPS compared to males of the control group and lower dosage of LHRHa. Analysis of variance showed no significant influence of the treatment on the velocity and percentage of motile spermatozoa. The effect of different treatment on the fertilization capacity (the number of spermatozoa per egg was equilibrated) was significant. Significantly the highest quality of sperm collected 72 h after injection expressed by percentage of fertilization and hatching (62-65% fertilization and 61-64% hatching rates, respectively) was found for LHRHa in dosage of 20 and 40 <EquationSource Format="TEX"><![CDATA[$$upmu$$]]></EquationSource> g kg⁻¹ b.w. Significantly the lowest parameters of fertilization and hatching were found for the control group, on the 12% level.

Keywords: Aquaculture; Fish; Hormone; Motility; Reproduction; Sperm; Spermatozoa; Tinca tinca

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s10499-005-9015-0

Affiliations: 1: Email: linhart@vurh.jcu.cz

Publication date: 2006-02-01

Related content

• In this: publication
• By this: publisher
• In this Subject: Aquaculture & Fisheries
• By this author: Caille, Nicolas ;  Rodina, Marek ;  Kocour, Martin ;  Gela, David ;  Flajšhans, Martin ;  Linhart, Otomar

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers