Inhibition of protease activities in discus Symphysodon spp. by three plant meals
Authors: Chong A.1; Hashim R.2; Bin Ali A.2
Source: Aquaculture International, Volume 10, Number 5, 2002 , pp. 433-441(9)
Publisher: Springer
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Abstract:
Based on biochemical assays and electrophoretical methods, the inhibitory effects of three plant meals (soybean meal, wheat meal, winged bean meal) on digestive alkaline proteases of discus were investigated. Casein assays revealed that increasing levels of soybean meal caused a linear inhibitory effect on activity of protease. SDS-PAGE images revealed that trypsin and chymotrypsin were the inhibited enzymes. Soybean showed the lowest inhibition rate followed by wheat meal and raw winged bean. There was a quadratic relationship between wheat meal levels and its inhibition of protease acitivity. The highest inhibitory effect was obtained with the winged bean meal with inhibition of caseinolytic activities ranging from 3.6–98.6%. Results from this study showed the potential of both soybean meal and wheat meal as ingredients for practical diet for discus, while demonstrating the need for further improvement in processing method for winged bean meal.
Keywords: Discus (Symphysodon spp.); Plant protein feedstuffs; Protease inhibition
Language: English
Document Type: Research article
Affiliations: 1: School of Biological Sciences, Universiti Sains Malaysia, Minden, 11800, Malaysia (e-mail: alex@usm.my) 2: School of Biological Sciences, Universiti Sains Malaysia, Minden, 11800, Malaysia
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