@article {Lundstrom:1997:1360-8185:189,
	author = "Lundstrom K.",
	author = "Pralong W.",
	author = "Martinou J-C.",
	title = "Anti-apoptotic effect of Bcl-2 overexpression in RIN cells infected with Semliki Forest virus",
	journal = "Apoptosis",
	volume = "2",
	year = "1997",
	abstract = "RIN cells were infected with recombinant Semliki Forest virus (SFV) particles containing the LacZ gene. X-gal staining showed 100&#037; infectivity of the cell cultures and high-level expression of bacterial <IMG SRC="/iso-ents/isogrk32/beta-s.gif" ALT="beta">-galactosidase in these cells. The cytopathogenic effects of the SFV infection were studied by measuring the viability of the RIN cells. Comparisons between control RIN cells and Bcl-2 overexpressing RIN cells were done 72 h post-infection. Significant differences in viability levels were observed. The control RIN cells showed in the MTT assay a mean value of 0.156&#177;0.017 compared to 0.347&#177;0.057 for the RIN/Bcl-2 cells. FACS analysis of cells labelled with propidium iodide indicated that only an average of 4.5&#177;0.5&#037; of the control cells were viable 72 h post-infection, while 44.5&#177;3.5&#037; of the RIN/Bcl-2 cells were still alive. Thus, the Bcl-2 overexpression clearly protected the SFV-infected cells from undergoing apoptosis.",
	pages = "189-191(3)",
	url = "http://www.ingentaconnect.com/content/klu/appt/1997/00000002/00000002/00172924"
}