Anti-apoptotic effect of Bcl-2 overexpression in RIN cells infected with Semliki Forest virus

Authors: Lundstrom K.1; Pralong W.2; Martinou J-C.1

Source: Apoptosis, Volume 2, Number 2, 1997 , pp. 189-191(3)

Publisher: Springer

Buy & download fulltext article:

OR

Price: $47.00 plus tax (Refund Policy)

Abstract:

RIN cells were infected with recombinant Semliki Forest virus (SFV) particles containing the LacZ gene. X-gal staining showed 100% infectivity of the cell cultures and high-level expression of bacterial beta-galactosidase in these cells. The cytopathogenic effects of the SFV infection were studied by measuring the viability of the RIN cells. Comparisons between control RIN cells and Bcl-2 overexpressing RIN cells were done 72 h post-infection. Significant differences in viability levels were observed. The control RIN cells showed in the MTT assay a mean value of 0.156±0.017 compared to 0.347±0.057 for the RIN/Bcl-2 cells. FACS analysis of cells labelled with propidium iodide indicated that only an average of 4.5±0.5% of the control cells were viable 72 h post-infection, while 44.5±3.5% of the RIN/Bcl-2 cells were still alive. Thus, the Bcl-2 overexpression clearly protected the SFV-infected cells from undergoing apoptosis.

Keywords: Anti-apoptosis; Bcl-2 expression; SFV infection

Document Type: Research article

Affiliations: 1: Glaxo Wellcome Research and Development, Geneva Biomedical Research Institute Plan-les-Ouates Switzerland 2: Modex Therapeuriques SA Lausanne Switzerland

Publication date: 1997-01-01

Related content

Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content

Text size:

A | A | A | A
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages. print icon Print this page