Optical Transillumination Tomography for Imaging of Tissue-Engineered Blood Vessels
Source: Annals of Biomedical Engineering, Volume 33, Number 3, January 2005 , pp. 323-327(5)
Abstract:Recent progress in tissue engineering led to the development of completely biological human vessels grown from the patient’s own cells. Those tissue-engineered blood vessels (TEBV) are grown on an individual basis at high costs per item, and therefore require close growth monitoring and quality control. We designed and tested an optical transillumination tomography system using red laser light to image weakly scattering specimens, such as TEBV. Fixated TEBV were imaged and the results compared to optical coherence tomography. This preliminary scanner prototype had an in-plane resolution of 50 m and allowed to see small inhomogeneities and defects in the samples. Tissue attenuation was found to be 70 cm−1. Main advantages of the transillumination tomography scanner over optical coherence tomography were the inexpensive instrumentation and the potential to rapidly acquire complete 3D sections with a CCD camera. The prototype presented in this study provides a basis to further improve image quality and acquisition speed.
Document Type: Research Article
Affiliations: 1: Department of Biological Engineering, University of Missouri-Columbia, USA, 2: Cytograft Tissue Engineering, Novato, CA, 3: Department of Biological Engineering, University of Missouri-Columbia, USA, Email: HaidekkerM@missouri.edu
Publication date: 2005-01-01