Authors: Hayden, Gavin¹; Milne, Helen¹; Patterson, Mark¹; Nimmo, Myra²

Source: European Journal of Applied Physiology, Volume 91, Numbers 5-6, May 2004 , pp. 748-751(4)

Publisher: Springer

Abstract:

Seven active male subjects cycled for 60 min at 29.5 (0.8)% peak work rate on three separate occasions in a hot environmental condition [36.0 (0.1)°C, 60 (1)% relative humidity] in order to determine the reproducibility of a closed-pouch sweat collection technique for sweat composition at the scapula, forearm and thigh. To confirm that sweat composition was not influenced by between-trial variations in sudomotor drive, local sweat rate, whole-body sweat rate, heart rate (HR), rectal temperature (T_re) and mean skin temperature (T_sk) responses were also measured, consequently reproducibility was also established for these variables. Sweat composition did not differ among trials, with the mean coefficients of variation (CVs) for sweat [Na⁺], [K⁺] and pH being 10.4 (7.4)%, 8.1 (6.5)% and 1.3 (1.1)%, respectively. Local sweat rates did not differ among the three trials (P>0.05) although whole-body sweat rate was reduced in the third trial (P<0.05). The mean CVs were 11.0 (7.8)% and 4.7 (1.6)% for local and whole-body sweat rates, respectively. Between-trial differences were not evident for T_re, T_sk or HR with mean CVs of 0.3 (0.2)%, 0.7 (0.6)% and 3.9 (1.7)%, respectively, although HR tended to be greater in the first trial (P=0.08). It is proposed that moderate variations in sweat composition were influenced by variations in the local sweat rate, which were induced by application of the pouch.

Keywords: Sweat collection; Thermoregulation; Reproducibility; Exercise; Heat

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s00421-004-1057-x

Affiliations: 1: Department of Applied Physiology, Faculty of Science, University of Strathclyde, 76 Southbrae Drive, Glasgow , G13 1PP, UK, 2: Department of Applied Physiology, Faculty of Science, University of Strathclyde, 76 Southbrae Drive, Glasgow , G13 1PP, UK, Email: m.a.nimmo@strath.ac.uk

Publication date: 2004-05-01

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