Neuronal apoptosis following human brain injury

Authors: Hausmann, R.¹; Biermann, T.²; Wiest, I.²; Tübel, J.²; Betz, P.²

Source: International Journal of Legal Medicine, Volume 118, Number 1, February 2004 , pp. 32-36(5)

Publisher: Springer

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Abstract:

Neuronal apoptosis has been investigated in paraffin-embedded brain tissue from 103 individuals who had sustained blunt head injury by use of the in situ nick translation (ISNT) technique. In order to provide reliable data for a forensic wound age estimation, a quantitative morphometric analysis was performed. Apoptotic neuronal cells could be detected in a cortical contusion with a wound age of 45 min at the earliest and in the majority of the cases with postinfliction intervals up to 2 weeks, numerous ISNT-positive cells were found adjacent to the traumatically injured area. The presented data indicate that neuronal apoptosis peaks at about 1 day and persists for at least 22 weeks after blunt head injury. The time-dependent occurrence of apoptotic cells can contribute to a forensic timing of cortical contusions and complements other immunohistochemical parameters, especially in the early postinfliction interval.

Keywords: Human brain injury; Apoptosis; Wound age

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s00414-003-0413-4

Affiliations: 1: Institute of Legal Medicine, University of Erlangen, Universitätsstrasse 22, 91054, Erlangen, Germany, Email: roland.hausmann@recht.imed.uni-erlangen.de 2: Institute of Legal Medicine, University of Erlangen, Universitätsstrasse 22, 91054, Erlangen, Germany,

Publication date: 2004-02-01