Determination of Phthalates in Diet and Bedding for Experimental Animals Using Gas Chromatography-Mass Spectrometry

Authors: Kondo, Fumio¹; Okumura, Masanao²; Oka, Hisao²; Nakazawa, Hiroyuki³; Izumi, Shun-ichiro⁴; Makino, Tsunehisa⁴

Source: Bulletin of Environmental Contamination and Toxicology, Volume 84, Number 2, February 2010 , pp. 212-216(5)

Publisher: Springer

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Abstract:

We have developed a gas chromatography-mass spectrometry method to measure five phthalates (dibutyl phthalate, butylbenzyl phthalate, di-2-ethylhexyl phthalate, diisooctyl phthalate, and diisononyl phthalate) in diets and beddings for experimental animals. The recoveries from diets and beddings spiked with five phthalates were 98.8%-148% with coefficients of variation of 0.4%-7.8% for diets and 94.7%-146% with coefficients of variation of 1.0%-5.0% for beddings. We analyzed commercial animal diets and beddings, and found that the levels of phthalates varied from sample to sample; the concentrations of five phthalates were 141-1,410 ng/g for diets and 20.5-7,560 ng/g for beddings.

Keywords: Phthalates; Animal diets; Beddings; Gas chromatography-mass spectrometry

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s00128-009-9919-x

Affiliations: 1: Department of Toxicology, Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya, 462-8576, Japan, Email: fumio@ipc-tokai.or.jp 2: Department of Toxicology, Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya, 462-8576, Japan 3: Department of Analytical Chemistry, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo, 142-8501, Japan 4: Department of Obstetrics and Gynecology, School of Medicine, Tokai University, Bohseidai, Isehara, 25-1193, Japan

Publication date: 2010-02-01