Development and genetic mapping of sequence-tagged microsatellites (STMs) in bread wheat (Triticum aestivum L.)

Authors: Hayden, M.; Stephenson, P.; Logojan, A.; Khatkar, D.; Rogers, C.; Elsden, J.; Koebner, R.; Snape, J.; Sharp, P.

Source: Theoretical and Applied Genetics, Volume 113, Number 7, November 2006 , pp. 1271-1281(11)

Publisher: Springer

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Abstract:

The density of SSRs on the published genetic map of bread wheat (Triticum aestivum L.) has steadily increased over the last few years. This has improved the efficiency of marker-assisted breeding and certain types of genetic research by providing more choice in the quality of SSRs and a greater chance of finding polymorphic markers in any cross for a chromosomal region of interest. Increased SSR density on the published wheat genetic map will further enhance breeding and research efforts. Here, sequence-tagged microsatellite profiling (STMP) is demonstrated as a rapid technique for the economical development of anonymous genomic SSRs to increase marker density on the wheat genetic map. A total of 684 polymorphic sequence-tagged microsatellites (STMs) were developed, and 380 were genetically mapped in three mapping populations, with 296 being mapped in the International Triticeae Mapping Initiative W7984 × Opata85 recombinant inbred cross. Across the three populations, a total of 479 STM loci were mapped. Several technological advantages of STMs over conventional SSRs were also observed. These include reduced marker deployment costs for fluorescent-based SSR analysis, and increased genotyping throughput by more efficient electrophoretic separation of STMs and a high amenability to multiplex PCR.

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s00122-006-0381-4

Affiliations: 1: Email: matthew.hayden@adelaide.edu.au

Publication date: 2006-11-01