Simple method of Arabidopsis thaliana cultivation in liquid nutrient medium

Authors: Siedlecka, Anna; Krupa, Zbigniew

Source: Acta Physiologiae Plantarum, Volume 24, Number 2, June 2002 , pp. 163-166(4)

Publisher: Springer

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Abstract:

Simple method of Arabidopsis thaliana w.t. cv. Columbia (L.) Heynh. cultivation in liquid nutrient medium is presented. After 5 weeks of growth in soil, the plants were transferred to modified Hoagland nutrient medium. This allowed us to cultivate Arabidopsis in conditions comparable to all other hydroponically grown higher plants used in plant physiology and plant stress physiology experiments. Absence of agar in growth medium and free access to whole root system makes this method useful also in experiments concerning root physiology.

Keywords: Arabidopsis thaliana; cultivation; liquid nutrient medium; method

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s11738-002-0007-7

Affiliations: 1: Email: Anna.Siedlecka@umcs.lublin.pl

Publication date: 2002-06-01