Somatic embryogenesis from chickpea (Cicer arietinum L.) inmature cotyledons: The effect of zeatin, gibberellic acid and indole-3-butyric acid
Authors: Hita, Oscar; Lafarga, Carmen; Guerra, Hilario
Source: Acta Physiologiae Plantarum, Volume 19, Number 3, September 1997 , pp. 333-338(6)
Abstract:Studies were conduced to test the effects of various cytokinins on somatic embryogenesis from chickpea (Cicer arietinum L.) immature cotyledons. Zeatin (13.7 µmol) added, to B5 basal medium, supplemented with 1.5 % sucrose and 0.2 µmol indole-3-acetic acid, was the most effective cytokinin. Lobular structures obtained from cotyledons cultures were transferred to B5 basal medium supplemented with gibberellic acid and indole-3-butyric acid at different concentrations. The most effective treatment was B5 medium containing 14.4 µmol gibberellic acid plus 1.0 µmol indole-3-butyric acid in which 42.8 % of lobular structures cultured formed normal somatic embryos. High conversion of embryos into plantlets (61.0–65.2 % embryos regenerated plants) was observed when germinated embryos were placed on plant development medium.
Document Type: Research Article
Publication date: September 1, 1997