Telomerase Activity in Giant Cell Tumors of Bone

Authors: Matsuo, Toshihiro¹; Hiyama, Eiso²; Sugita, Takashi³; Shimose, Shoji⁴; Kubo, Tadahiko⁴; Mochizuki, Yu⁴; Adachi, Nobuo⁴; Kojima, Kensaku⁵; Sharman, Patrick⁴; Ochi, Mitsuo⁴

Source: Annals of Surgical Oncology, Volume 14, Number 10, October 2007 , pp. 2896-2902(7)

Publisher: Springer

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Abstract:

A giant cell tumor of bone (GCT) is a histologically benign neoplasma that has an unpredictable pattern of biological aggressiveness. In the present study, we investigated whether there was a correlation between telomere length or the levels of telomerase activity and other clinical features of GCTs, for the possible use of these factors as parameters of aggressiveness or prognosis.

In 16 surgically resected GCTs specimens, telomere length was assessed by terminal restriction fragments by Southern blot analysis. Telomerase activity was measured by a semiquantitative polymerase chain reaction-based telomeric repeat amplification protocol assay.

Telomere length reduction was observed in 69% of the GCT samples. The telomere lengths of tumors were significantly shorter than those of normal tissue (P = .008). The mean telomere length of grade 3 tumors was significantly shorter than those of grade 1 and 2 tumors (P = .038). Telomerase activity was detected in 81% of tumor samples. The level of telomerase activity in tumors with local recurrence was significantly higher than in tumors without local recurrence (P = .011).

These results suggest that telomere length correlates with roentgenographic grade as a result of the frequency of cell division, and high telomerase activity indicates the aggressiveness of GCTs.

Keywords: Telomere length; Telomerase activity; Giant cell tumor of bone; Clinical marker; Aggressiveness

Document Type: Research article

DOI: 10.1245/s10434-007-9391-y

Affiliations: 1: Department of Orthopaedic Surgery, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan, Email: tomatsuo@hiroshima-u.ac.jp 2: Natural Science Center for Basic Research and Development, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan 3: Department of Orthopaedic Surgery, Hiroshima Prefectural Hospital, 1-5-54 Ujinakanda, Minami-ku, Hiroshima, 734-8530, Japan 4: Department of Orthopaedic Surgery, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan 5: Department of Surgery, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan

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