Application of hot water emasculation to Acacia auriculiformis for controlled pollination
Source: Journal of Forest Research, Volume 15, Number 3, June 2010 , pp. 210-216(7)
Abstract:To determine the viability of hot water emasculation for Acacia auriculiformis, spikes composed of about 100 flowers were soaked in hot water at varying temperatures (45–60°C) and for varying lengths of time (5 s to 7 min) prior to anthesis. The rate of squashed polyads, each of which consisted of 16 pollen grains, and the germinating rate on media, decreased with temperature and time. A mating test suggested that the rates of adhering polyads per stigma and germinating polyads per stigma were lower in the self-pollination of soaked spikes than in that of normal spikes. On the other hand, the number of matured pods was not significantly different in the mating between the spikes receiving the soaking treatment (♀) and the spikes receiving no treatment (♂) compared with the mating between normal spikes. Although further research is needed to determine the optimal treatment conditions, these results suggest that soaking spikes into hot water, especially at 50°C for 45 s, 54°C for 15 s, and 56°C for 10 s, is an effective emasculation technique.
Document Type: Research Article
Affiliations: 1: Iriomote Tropical Forest Tree Breeding Technical Garden, Forest Tree Breeding Center, Forestry and Forest Products Research Institute, 3809-1 Ishi, Juo, Hitachi, Ibaraki, 319-1301, Japan 2: Iriomote Tropical Forest Tree Breeding Technical Garden, Forest Tree Breeding Center, Forestry and Forest Products Research Institute, 3809-1 Ishi, Juo, Hitachi, Ibaraki, 319-1301, Japan, Email: firstname.lastname@example.org
Publication date: June 1, 2010