Systematic identification and molecular characterization of genes differentially expressed in breast and ovarian cancer

Authors: Edgar Dahl¹; Ariane Sadr-Nabavi²; Eva Klopocki³; Beate Betz⁴; Susanne Grube⁵; Rene Kreutzfeld⁶; Marina Himmelfarb¹; Han-Xiang An¹; Stephen Gelling³; Irina Klaman³; Bernd Hinzmann³; Glen Kristiansen⁷; Robert Grützmann⁸; Ruprecht Kuner³; Beate Petschke³; Kerstin Rhiem⁶; Kai Wiechen⁷; Christine Sers⁷; Otmar Wiestler⁹; Achim Schneider⁵; Heinz Höfler¹⁰; Jörg Nährig¹⁰; Manfred Dietel⁷; Reinhold Schäfer⁷; André Rosenthal³; Rita Schmutzler⁶; Matthias Dürst⁵; Alfons Meindl²; Dieter Niederacher⁴

Source: The Journal of Pathology, Volume 205, Number 1, January 2005 , pp. 21-28(8)

Publisher: John Wiley & Sons, Ltd.

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Abstract:

The identification of novel disease-associated genes in gynaecological tumours has important implications for understanding the process of tumourigenesis and the development of novel treatment regimens. cDNA libraries from disease tissues may represent a valuable source to identify such genes. Recently, a bio-informatic procedure based on an ‘electronic Northern’ approach was established to screen expressed sequence tag (EST) libraries for genes differentially expressed in tumour and normal tissues, and identified 450 candidate genes differentially expressed in breast and ovarian cancer. In this report, the validation of an initial set of 40 candidate genes, which were selected due to their localization in chromosomal regions frequently altered in gynaecological tumours, is described. Differential expression of 29 of these genes, including three uncharacterized novel genes, was confirmed by applying cancer profiling arrays with 106 matched pairs of tumour/normal cDNAs and quantitative reverse transcription-polymerase chain reaction (RT-PCR) on 60 clinical specimens. The majority of these differentially expressed genes have not been described previously in the context of breast and ovarian cancer, and may constitute novel diagnostic markers for these tumour entities. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

Keywords: breast cancer; ovarian cancer; electronic Northern; differentially expressed gene

Document Type: Research article

DOI: 10.1002/path.1687

Affiliations: 1: Institute of Pathology, RWTH Aachen, Germany 10: Institute of Pathology, Technical University of Munich, Germany 2: Medical Genetics, Department of Pediatric LMU Munich, Germany 3: metaGen Pharmaceuticals iL, Berlin, Germany 4: Department of Gynecology and Obstetrics, Heinrich-Heine-University, Duesseldorf, Germany 5: Department of Gynecology, Friedrich-Schiller-University, Jena, Germany 6: Department of Gynecology, Medical Center, University of Cologne, Germany 7: Institute of Pathology, University Hospital Charité, Humboldt-University, Berlin, Germany 8: Department of Visceral, Thoracic and Vascular Surgery, University Hospital, Technical University Dresden, Germany 9: Department of Neuropathology, University of Bonn, Germany

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