Skip to main content

Technical note on a much simplified method for collecting ruminal fluid using a nylon paint strainer

The full text article is not available for purchase.

The publisher only permits individual articles to be downloaded by subscribers.

Collection of ruminal fluid for in vitro experimentation has traditionally been accomplished by straining rumen digesta through multiple layers of cheesecloth or other cumbersome filtering materials, such as surgical gauze, nylon tissues or wire gauzes. We here report a comparison of the gross physical, fermentative and microbial characteristics of ruminal fluid collected by straining through cheesecloth or through a nylon mesh paint strainer that, because of its pouched shape, is conveniently filled with digesta thus making the collection and straining process much easier. Whether strained through cheesecloth or the nylon mesh strainer, ruminal fluid did not differ (P > 0.05) in dry matter, neutral or acid detergent fiber content or in concentrations of total culturable anaerobes. Total amounts (mM) of volatile fatty acid or ammonia produced after 24 h of incubation of cheesecloth‐ or nylon mesh‐strained ruminal fluid with added tryptose, SigmaCell 50 or starch did not differ (P > 0.05) thus indicating that the microbial populations within the strained fluids possessed similar abilities to ferment these test substrates. Because the physical, fermentative and microbial characteristics of the nylon mesh‐ and cheesecloth‐strained ruminal fluid were essentially equivalent, we conclude that the much easier to use nylon mesh strainers can be incorporated into a faster, more convenient ruminal fluid straining method. Published in 2004 for SCI by John Wiley & Sons, Ltd.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: bovine; fermentation; ruminal fluid; strainers

Document Type: Research Article

Affiliations: United States Department of Agriculture, Agricultural Research Service, Food & Feed Safety Research Unit, Southern Plains Agricultural Research Center, College Station, TX 77845, USA

Publication date: 2004-03-01

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more