Supercritical CO₂ extraction of Chilean hop (Humulus lupulus) ecotypes

Authors: del Valle J.M.¹; Rivera O.¹; Teuber O.²; Palma M.T.²

Source: Journal of the Science of Food and Agriculture, Volume 83, Number 13, October 2003 , pp. 1349-1356(8)

Buy & download fulltext article:

The full text article is not available for purchase.

The publisher only permits individual articles to be downloaded by subscribers.

Abstract:

Hops (Humulus lupulus L) have very stringent requirements of diurnal light hours during flowering. Thus, the beer industry is permanently assessing new agricultural land to grow hops, southern Chile being an excellent candidate for their introduction. Supercritical carbon dioxide extraction procedures were implemented to assess Chilean hop ecotypes. Based on results of a preliminary study with commercial samples (cv Nugget), 200 bar and 40 °C were selected as extraction conditions. Very limited increases in extraction rate were observed when applying pressures >200 bar; such pressures did not speed up the extraction of alpha

-acids (bitter flavor principles), but rather increased the co-extraction of undesirable compounds (hard resins and chlorophyll pigments). On the other hand, the effects of retrograde condensation phenomena were just starting to subside at 200 bar, and thermal damage of samples could increase at >40 °C. Ground cone samples of five local hop ecotypes, originating from Osorno, La Junta, Coyhaique and Elizalde Lake, were subsequently tested. Osorno exhibited a higher yield (65 g kg^-1 oleoresin/substrate) than other Chilean ecotypes. Moreover, Osorno extract had a light yellowish color, as well as a higher content of bitter flavors (157 g kg^-1 alpha

-acid/extract) and 1.5–7.1 times more representative aroma compounds than extracts of the other ecotypes. However, all these indicators were very low compared with the commercial Nugget sample (134 g kg^-1 oleoresin/substrate, 383 g kg^-1 alpha

Keywords: supercritical fluid extraction; carbon dioxide; hop cones; soft resins; alpha -acids; essential oils

Document Type: Research article

DOI: http://dx.doi.org/10.1002/jsfa.1547

Affiliations: 1: Extraction Laboratory of Biological Materials, Chemical and Bioprocess Engineering Department, Pontificia Universidad Católica de Chile, Avda Vicuña Mackenna 4860, Macul, Santiago, Chile 2: Regional Research Center Tamel Aike, Instituto de Investigaciones Agropecuarias, Coyhaique, Chile

Publication date: 2003-10-01

More about this publication?

As of January 2010, this journal will no longer be available on IngentaConnect, please visit Wiley InterScience to arrange continued access to this content
Access this journal at Wiley InterScience
ingentaconnect is not responsible for the content or availability of external websites