Optimization of polymerase chain reaction-amplified conditions using the uniform design method

Authors: Yang Cao; Yuanyuan Zheng; Baishan Fang

Source: Journal of Chemical Technology & Biotechnology, Volume 79, Number 8, August 2004 , pp. 910-913(4)

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Abstract:

Polymerase chain reaction (PCR) is a key procedure in modern molecular biology but may be adversely affected by many factors and it is difficult to optimize reaction conditions; a technically simple and effective method to set up an optimal PCR-amplified system is required. Uniform design (UD) is a method that enables many factors to be investigated. For example, the dhaT gene from Klebsiella pneumoniae was amplified and the optimization of the amplified parameters including concentration of Mg²⁺, annealing temperature, annealing time and number of cycles based on UD was reported. After only 12 experimental trials, an optimal PCR-amplified result was obtained, demonstrating the value of UD for optimization of PCR. Copyright © 2004 Society of Chemical Industry

Keywords: dhaT gene; optimization; PCR; uniform design

Document Type: Research article

DOI: http://dx.doi.org/10.1002/jctb.1078

Affiliations: 1: Department of Bioengineering and Biotechnology, Huaqiao University, 362011, Quanzhou, Fujian, China

Publication date: 2004-08-01

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