Oestrogen inhibits human colonic motility by a non-genomic cell membrane receptor-dependent mechanism

Authors: Hogan, A. M.; Kennelly, R.; Collins, D.; Baird, A. W.; Winter, D. C.

Source: British Journal of Surgery, Volume 96, Number 7, July 2009 , pp. 817-822(6)

Publisher: John Wiley & Sons, Ltd.

Abstract:

Background:

Classical effects of oestrogen involve activation of target genes after binding nuclear receptors. Oestrogenic effects too rapid for DNA transcription (non-genomic) are known to occur. The effect of oestrogen on colonic motility is unknown despite the prevalence of gastrointestinal symptoms in pregnant and premenopausal women.

Methods:

Histologically normal colon was obtained from proximal resection margins of colorectal carcinoma specimens. Circular smooth muscle strips were microdissected and suspended in organ baths under 1 g of tension. After equilibration, they were exposed to 17β-oestradiol (n = 8) or bovine serum albumin (BSA)-conjugated 17β-oestradiol (n = 8). Fulvestrant, an oestrogen receptor antagonist, was added to some baths (n = 8). Other strips were exposed to calphostin C or cycloheximide. Carbachol was added in increasing concentrations and contractile activity was recorded isometrically.

Results:

Oestrogen inhibited colonic contractility (mean difference 19·7 per cent; n = 8, P < 0·001). In keeping with non-genomic, rapid-onset steroid action, the effect was apparent within minutes and reversible. It was observed with both 17β-oestradiol and BSA-conjugated oestrogen, and was not altered by cycloheximide. Effects were inhibited by fulvestrant, suggesting receptor mediation.

Conclusion:

Oestrogen decreases contractility in human colonic smooth muscle by a non-genomic mechanism involving cell membrane coupling. Copyright © 2009 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.

Document Type: Research article

DOI: http://dx.doi.org/10.1002/bjs.6612

Publication date: 2009-07-01

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