MicroRNA Expression Profile in Hyperoxia-Exposed Newborn Mice During the Development of Bronchopulmonary Dysplasia
Authors: Zhang, Xiaoying; Peng, Wei; Zhang, Sheng; Wang, Chunzhi; He, Xiyu; Zhang, Zhimei; Zhu, Lina; Wang, Yan; Feng, Zhichun
Source: Respiratory Care, Volume 56, Number 7, July 2011 , pp. 1009-1015(7)
Publisher: The Journal Respiratory Care Company
Abstract:BACKGROUND: Bronchopulmonary dysplasia (BPD) is a chronic lung disease of preterm neonates; the underlying pathogenesis is not fully understood. MicroRNAs (length 21‐25 nucleotides) are ribonucleic acid (RNA) molecules that have important functions in development, cellular differentiation, apoptosis, proliferation, and migration; very little is known regarding their role in developmental lung diseases. METHODS: We exposed neonatal mice to either room air or 60% oxygen, beginning at birth, and we used microRNA microarray and real-time polymerase chain reaction on lung samples. RESULTS: The hyperoxia-exposed mice developed a lung injury that mimicked human BPD. Fifty-one microRNAs shared similar profiles in the hyperoxia-exposed BPD lungs and the normal lungs, which indicates that those microRNAs might play a protective role during the septation process. In the BPD lungs, compared to the control lungs, 14 microRNAs were up-regulated, and 7 microRNAs were down-regulated, which indicates that these microRNAs might play an important role in the development of BPD. Some of the candidate microRNAs can regulate cell proliferation. CONCLUSIONS: To our knowledge, this study is the first to identify microRNAs associated with BPD development, which provides a clue for further investigation of their function in BPD development.
Document Type: Research Article
Affiliations: Department of Pediatrics, BaYi Children’s Hospital of The General Military Hospital of Beijing People’s Liberation Army, Beijing, People’s Republic of China.
Publication date: 2011-07-01
- As of January 1, 2013, Respiratory Care content will no longer be hosted on ingentaconnect. Please contact the publisher at firstname.lastname@example.org for information on how to continue access to this title.
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- Clinical Practice Guidelines
- Past OPEN FORUM Abstracts
- ingentaconnect is not responsible for the content or availability of external websites