Expression analysis and subcellular localization of wheat aluminum-induced gene TaAIP
TaAIP was obtained as the interaction protein of wheat stress-related TaMAPK2, which was used as bait protein to screen the wheat cDNA library by yeast-two-hybrid system. TaAIP, containing a wali domain, was similar to some aluminum-induced proteins. Real-time PCR showed that the expression
of TaAIP was up-regulated by the imposition of aluminum, high-salt and drought stress. Semi-quantitative RT-PCR analysis indicated TaAIP was a stem-specific gene, not expressed in root, leaves and flower. The subcellular localization assay indicated that TaAIP located on plasma membrane. These
results provided the foundation for further analysis of TaAIP resistance mechanisms.
Keywords: expression profile; stress; subcellular localization; wheat; yeast-two-hybrid
Document Type: Research Article
Publication date: 15 January 2014
- Journal of plant genetic resources is devoted to reporting new and original experimental and theoretical basic research of interest to genetic resources, gene mining, brief information,and germplasm innovation. Manuscripts that are essentially reporting data, applications of data, or reviews of the literature are not suitable for publication in Journal of plant genetic resources.
Journal of plant genetic resources was founded in 2000 and is sponsored by the institute of crop sciences of chinese academy of agriculture sciences and China association of agricultural science societies. Since 2003, Journal of plant genetic resources has been indexed in China scientific journal database (CSJD), Chinese core journals (selection) database,Chinese science citation database (CSCD), and so on. - Editorial Board
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