Simultaneous assay of isoflavones in Fructus Sophorae by QAMS
Objective: To establish a method for the determination of the isoflavone content in Fructus Sophorae by quantitative analysis of multi-components by single marker (QAMS). Methods: The relative correction factors (RCFs) of sophorabioside,genistin and genistein to that of
sophoricoside were calculated on an Agilent Extend C18 column (250 mm×4.6 mm,5 μm) with methanol-acetonitrile-0.1% phosphoric acid aqueous solution as the mobile phase.The flow rate was 1.0 mL·min-1,the column temperature was set at 30°C,and the detector
wavelength was 260 nm.The relative retention time (RRT) was used to define the target peak. Results: Sophoricoside,sophorabioside,genistin,and genistein showed a good linear relationship within ranges of 0.374-7.49 μg,2.16×10-2-0.432 μg,2.61×10-2-0.522
μg,and 1.02×10-2-0.203 μg,respectively,and the recoveries were 100.7%,96.3%,98.6%,and 100.8%,respectively.The RCFs of sophorabioside,genistin and genistein were 0.80,1.03,1.61,and the RRTs were 0.42,0.81,1.26,respectively.Conclusion: The QAMS method is simple,rapid,accurate
and reliable,and it can be applied to determine the content of isoflavones in Fructus Sophorae.
PLEASE NOTE: The full text of this publication is in Chinese.
Keywords: QAMS; genistein; genistin; related correlation factor (RCF); relative retention time (RRT); sophorabioside; sophoricoside
Document Type: Research Article
Affiliations: 1: Shenyang Pharmaceutical University Shenyang 110016 China 2: Zhoushan Municipal Institute for Food and Drug Control Zhoushan 316021 China
Publication date: 01 January 2013
- Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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