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Two reference substances for determination of multiple components ():linear calibration using two reference substances for identification of chromatographic peaks

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Objective: To establish a new substitute reference substance method named as "two reference substances for determination of multiple components(TRSDMC)" for multi-components assay of rhubarb and its preparations. Methods: The peak area and retention time of the five free anthraquinones in rhubarb were determined on five different HPLC instruments with 14 C18 columns of different brands and types,then the data analysis was conducted.For the quantitative analysis,aloe-emodin was used as the reference substance for determination of relative correction factors of rhein,emodin,chrysophanol and physcion.For the qualitative analysis,both aloe-emodin and physcion were served as linear calibration standards to forecast the retention time of the other three components. Results: In quantitative aspect,the relative correction factors determined were stable in a certain variation range of column temperature,flow rate and proportion of the mobile phase.In qualitative aspect,linear calibration using two reference substances was proved to be more accurate than the relative retention time method for chromatographic peak identification by validation tests with 14 columns. Conclusion: It is demonstrated that TRSDMC is practical and promising for multi-component assay of traditional Chinese medicine.

PLEASE NOTE: The full text of this publication is in Chinese.

Keywords: high-performance liquid chromatography; linear calibration using two reference substances(LCTRS); quantitative analysis of multi-components by single marker(QAMS); rhubarb; substitute reference substance(SRS); two reference substances for determination of multiple components(TRSDMC)

Document Type: Research Article

Publication date: 01 January 2013

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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