HPLC method for simultaneous measurement of seven water-soluble ingredients with single marker in Danshen injection
Objective:To establish a new quantitative method for simultaneous determination of multi-component(sodium danshensu,protocatechuic acid,protocatechuic aldehyde,vanilla acid,ferulic acid,rosmarinic acid,salvianolic acid B) in Danshen injection by using one chemical reference substance,and
to validate its feasibilities. Methods:The quantitative analysis of multi-components by single-marker(QAMS) was established and validated with Danshen injection.Seven main ingredients were evaluated and their relative correlation factors(RCFs)were determined by HPLC-UV.The contents
of protocatechuic aldehyde in the samples of Danshen injection were calculated by using the external standard method,and the contents of the six other ingredients were calculated by their RCFs.The contents of these six ingredients in all samples were also determined by using the external standard
method. Results:The RCFs of sodium danshensu,protocatechuic acid,vanilla acid,ferulic acid,rosmarinic acid and salvianolic acid B all showed good reproducibility within certain ranges.For sodium danshensu,protocatechuic acid,vanilla acid,ferulic acid,rosmarinic acid and salvianolic
acid B,there was no significant difference between the quantitative results of the two methods. Conclusions:The method is simple,accurate and suitable to evaluate the contents of seven main water-soluble substances in Danshen injection.
PLEASE NOTE: The full text of this publication is in Chinese.
Keywords: Chinese materia medica preparation multi-component analysis; HPLC; QAMS; RCF; Radix et Rhizoma Salviae Miltiorrhizae injection; ferulic acid; methodological inspection; phenolic acids; protocatechuic acid; protocatechuic aldehyde; rosmarinic acid; salvianolic acid B; sodium danshensu; vanilla acid; water-soluble substance
Document Type: Research Article
Affiliations: 1: Lanzhou University Lanzhou 730000 China 2: Gansu Institute for Food and Drug Control Lanzhou 730000 China
Publication date: 01 January 2012
- Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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