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Enantiomeric resolution of citadiol on immobilized cellulose chiral stationary phase

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Objective: To establish an HPLC method for the chiral separation of citadiol(CTD) enantiomers to determine the optical purity of products using different separation processes. Methods: The column used was Chiralpak IC(250 mm×4.6 mm,5 μm) at a flow rate of 1.0 mL·min-1 and 25 .Effects of standard mobile phases including the bulk of hexane and nonstandard mobile phases involving tetrahydrofuran,methyl tertiary butyl ether,methylene chloride,etc.were studied on the chiral separation of CTD.Effects of temperature and the system applicability test were investigated using the optimal mobile phases. Results: The optimal standard mobile phase consisted of hexane-n-propanol-TEA(80:20:0.1,v/v) with the resolution value of 9.04,while the optimal nonstandard one was hexane-CH2Cl2-ethanol-DEA(50:50:2:0.1,v/v) with the resolution value of 10.22.S-(-)-CTD eluted prior to R-(+)-CTD. Conclusion: Using the optimal mobile phases CTD enantiomers can be separated in short time,with high resolution values and good system applicability.These methods can be used to determine the optical purity of CTD with high efficiency and good repetition.

PLEASE NOTE: The full text of this publication is in Chinese.

Keywords: HPLC; chiral stationary phase; citalopram; enantiomeric separation; midbody; mobile phases optimization; optical purity determination

Document Type: Research Article

Publication date: 01 January 2011

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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