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Determination of the amount of thiol anti-oxidant in hemoglobin- based oxygen carrier with o-phenanthroline-Fe3+ *

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Objective: To establish a method for determination of thiol anti-oxidant in Hemoglobin-Based Oxygen carrier (HBOC). Method: It is found that polymerized hemoglobin in HBOC can absorb the thiol anti-oxidant which is difficult to be separated,so taking the hemoglobin as the control and o-phenanthroline-Fe3+ as developer and oxidant,direct determining the content of cysteine,glutathion and N-acetyl-cysteine in HBOC at 510 nm. Results: all the calibration curves showed good linearity in the range of 1×10-6-1.5×10-5 mol·L-1,and the correlation coefficient are 0.9991,0.9989 and 0.9985,all of them have the good precision,and the recovery are 97.3%±3.5%,96.7%±4.1% and 95.5%±4.3%,respectively. Conclusion: This method avoided separating the thiol anti-oxidant from the HBOC,and can direct determine the contents of thiol anti-oxidant.It is a simple,accurate,high recovery method.

PLEASE NOTE: The full text of this publication is in Chinese.

Keywords: acetylcysteine; cysteine; glutathione; hemoglobin based oxygen carrier(HBOC); o-phenanthroline-Fe

Document Type: Research Article

Affiliations: 1: College of Life Science Northwest University Xi'an 710069 China 2: Shaanxi Lifegen Co. Ltd. Xi'an 710069 China

Publication date: 01 January 2011

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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