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Analysis of sugars and sugar alcohols in freeze-dried Dan-Shen injection

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Objective: To establish a method for the determination of sugars and sugar alcohols in freeze-dried Dan-Shen injection. Methods: 1-Methylimidazole was used as solvent and catalyst,hydroxylamine hydrochloride and acetic anhydride were used as reagent for oximization and acetylation. The products of the derivation reaction were separated effectively by gas chromatography with a capillary column, and structurally identified and characterized by GC-MS. Results: The structures of 8 sugars and sugar alcohols were identified and characterized by GC-MS in freeze-dried Dan-Shen injection, the content of six of them were determined. The calibration curve showed a good linear(r≥0.9995). The average recoveries were within 95.77%-104.4%. Conclusion: The method is simple, accurate, reliable and applicable for the determination of sugars and sugar alcohols in freeze-dried Dan-Shen injection.

PLEASE NOTE: The full text of this publication is in Chinese.

Keywords: GC; MS; Radix Salviae Miltiorrhizae; injection; qualitative; quantitative analysis; sugar alcohols; sugars

Document Type: Research Article

Affiliations: 1: Guangxi Institute for Food and Drug Control Nanning 530021 China 2: National Institutes for Food and Drug Control Beijing 100050 China 3: Harbin Pharm Group Co. LTD Second Chinese Medicine Factory Harbin 150078 China

Publication date: 01 January 2011

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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