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LCMS determination of diosgenin in rat plasma

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Abstract ObjectiveTo develop an HPLCMS method for the determination of diosgenin in rat plasma.
MethodsAdd the standard of Tanshinone A into the plasma samples ,then add some acid into the samples . The plasma samples were extracted with ethyl acetate. The ALLTIMA-C18(3.5µm,2.1mm×150mm) column was adopted. The mobile phase was consisted of acetonitrile–water including 0. 1 % trifluoroacetic acid ( TFA) (95:5 ). Electrospray ionization(ESI) and selected ion mass(SIM) were used to determine the diosgenin([M+H]+,m/z415.4) and Tanshinone IIA[M+H]+,m/z295.2 . ResultsThe linear range of Diosgenin was 1 to 100µg·ml-1. The recovery was above 70%. The intra and inter day variances were less than 16 %.
Conclusion This method is sensitive, specific and rapid. Therefore, it can be used for pharmacokinetic study of Diosgenin.

PLEASE NOTE: The full text of this publication is in Chinese.

Keywords: HPLCMS; Key words: diosgenin; concentration in plasm

Document Type: Research Article

Affiliations: College of Chemical Engineering Sichuan University

Publication date: 01 January 2009

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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