Studies on the components of unprocessed and processed Radix et Rhizoma RheiCortex Phellodendri and Radix Paeoniae Rubra by HPLC-UV and ESI-MS
Abstract Objective:To analyze the main chemical components existed in the unprocessed and processed Radix et Rhizoma Rhei,Cortex Phellodendri and Radix Paeoniae Rubra.Methods:The analysis was carried out by ESI-MS and HPLC-UV methods.MSelectrospray
ionization source,heated capillary temperature of 200 ,spray voltage of 4.5 kV LCAgilent Zorbax C18 column(4.6 mm×150 mm,5 μm),column temperature 28 .Results:The contents of chemical components in Radix et Rhizoma Rhei were different between samples processed
with wine and vinegar The content of palmatine in Cortex Phellodendri was increased and the content of berberine was decreased in sample processed with wine,but there was no difference between Cortex Phellodendri unprocessed and processed with salt The content of paeoniflorin was decreased
in processed Radix Paeoniae Rubra.Conclusion:Different processed methods are making a certain different influence on the contents of chemical components in Radix et Rhizoma Rhei,Cortex Phellodendri and Radix Paeoniae Rubra.
PLEASE NOTE: The full text of this publication is in Chinese.
Keywords: Radix et Rhizoma Rhei Cortex Phellodendri Radix Paeoniae Rubra processing technique ESI-MS HPLC-UV
Document Type: Research Article
Affiliations: (1.Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China 2.Jilin Agriculture University Changchun 130118 China)
Publication date: 01 January 2009
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