HPLC-MSTOF determination of ramiprilat in human plasma by solid-phase extraction
To establish a sensitive and specific liquid chromatography-mass spectrometry(time-of-fly)\[LC-MS(TOF)\]method for the determination of ramiprilat in human plasma.Method:Ramiprilat and the internal standard quinapril were extracted from the human plasma using SPE column,then separated
on a Symmetry C18 column.The mobile phase consisted of methanol-acetic acid(47:53,v/v),and was set at a flow rate of 0.3 mL·min-1.Detection is performed on a time-of-flight mass spectrometer equipped with an ESI interface and operated in positive-ionization mode.Ramiprilat quantity
was realized by computing the peak area ratio(ramiprilat m/z 389/ quinapril m/z 439)and comparing them with calibration curve(r=0.9995).Results:The linear calibration curve was obtained in the concentration range of 0.5-40 ng·mL-1.The low limit of quantitative was 0.5 ng·mL-1.Within-day
and between-day precisions were less than 10%.Conclusion:The method is sensitive,simple and rapid for drug level monitoing in clinical pharmacokinetics study.
PLEASE NOTE: The full text of this publication is in Chinese.
Keywords: HPLC-MS(TOF); human plasma concentration; ramipril; ramiprilat
Document Type: Research Article
Publication date: 01 February 2006
- Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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