HPLC Determination of Two Related Substancesin Azathioprine Tablets
An HPLC method was developed to determine 6-mercaptopurine and 5-chloro-1-methyl-4-nitroimidazolewhich were related substances of azathioprine tablets.MethodsAn ODS C18 column(250 mm×4.6 mm5 μm)was used with the mobile phase of a mixture of sodium heptanesulfonate solution(sodium
heptanesulfonate 1.336 gdissolved in 850 mL water)-methanol(85:15adjust the solution with 1 mol·L-1 hydrochloric acid to a pH of 3.5)at a flow rate of 1.0 mL·min-1The detection wavelength was at 254 nm.ResultsFor 6-mercaptopurinethe calibration curve was linear in the range
of 4.68×10-4-0.014 mg·mL-1 with correlation coefficient 0.9997 and the regression equation being Y=5400X-0.5302the average recovery(n=9)was 99.5%and the detection limit was 1 ngFor 5-chloro-1-methyl-4-nitroimidazolethe calibration curve was also linear in the range of 5.51×10-4-0.0165
mg·mL-1 with correlation coefficient 0.9994 and the regression equation being Y=6743X-1.396the average recovery(n=9)was 99.7%and the detection limit was 2.5 ng.Sample solution and standard solution are all stable within 8 h.ConclusionThis method is specificaccuratesensitive and simple.
PLEASE NOTE: The full text of this publication is in Chinese.
Keywords: 5-chloro-1-methyl-4-nitroimidazole; 6-mercaptopurine; HPLC; azathioprine tablets
Document Type: Research Article
Publication date: 01 September 2005
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