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Establishment of Fingerprint of Lingzhi(Ganoderma lucidum Karst.)and Its Preparations by HPLC

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To study the determination method of the fingerprint of lingzhi(Ganoderma lucidum)and its preparations and to establish the fingerprint of lingzhi and its preparations.Methods:The methanol extraction of lingzhi and its preparations was separated on a ZORBAX×300SB-C18column(4.6 mm×250 mm,5 μm)elution using MeOH and 5%Hac-H2O(40:60)solvent system and detected at 252 nm with a DAD detector.Flow rate was 0.8 mL·min-1 and 10 μL was injected every time and the column temperature was 40.Results:The fingerprint of lingzhi and its preparations with 12 common peaks was established.The relative retention time was determined.Conclusion:The fingerprint can be used to distinguish lingzhi preparations.This method is quicksimplerepeatableexact and need not any pretreatment.Therefore it provides scientific reference and effective determining ways for the quality control of lingzhi preparations.
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Keywords: HPLC; fingerprint; lingzhi(Ganoderma lucidum Karst.)

Document Type: Research Article

Publication date: 2005-03-01

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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