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The Inhibitory Effect of Matrine on Human Hepatitis Bvirus Proliferation in vitro

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To observe the effect of matrine on anti-proliferation of human hepatitis B virus(HBV)in vitro.Method:HepG2 2.2.15 cells(human HepG2 cells transfected with HBV genome)were cultured into 24 well culture plates with 0.025 to 0.5 mg·mL-1 matrine for 9 days.The concentration of HBsAg and HBeAg in the culture supernatant were determined by ELISA assay,the intracellular and intercellular HBV DNA were extracted and measured by fluorescence quantity PCR assay.Result:When administrated for 9 days,matrine within range of 0.025 to 0.5 mg·mL-1 produced dose-dependent inhibition of HBsAg and HBeAg excreted by HepG2 2.2.15 cells,with a 50% inhibitory concentration(IC50)of 0.14 mg·mL-1 and 0.29 mg·mL-1,respectively,and selectivity index(SI)were 5.66 and 2.69,respectively.Matrine within range of 0.25 to 0.5 mg·mL-1 reduced intracellular and intercellular HBV DNA replication in HepG2 2.2.15 cells,with IC50 of 0.109 mg·mL-1 and 0.120 mg·mL-1,respectively,and SI were 7.0 and 6.39,respectively.Conclusion:Results of the experiments in vitro indicate that Matrine within range of 0.25 to 0.5 mg·mL-1 has a dose-dependent inhibitory effect on proliferation of human hepatitis B virus and blocks the transcription of HBV in HepG2 2.2.15 cells.
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Keywords: HBV; HBeAg; HBsAg; HepG2 2.2.15 cells; matrine

Document Type: Research Article

Publication date: 2005-03-01

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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