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HPLC Determination of Content and Entrapment Efficiencyof Lomustine in Liposome

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To establish a RP-HPLC method for content and entrapment efficiency determination of Lomustine liposomes. Method:The separation was performed with a DiamonsilTM C18 Column(250 mm 4.6 mm,5 μm)and the mobile phase was acetonitrile-0.05mol·L-1ammonium dihydrogen phosphate solution-triethylamine(60:40:0.2pH was adjusted to 3.5 using phosphoric acid)flow rate was 1.0 mL·min-1 and lomustine was detected at 232 nma room column temperature.Result:Lomustine had a good linear relation in the range of 4.0-48.0 μg·mL-1(r=1.0n=5)the intra-day RSD and inter-day RSD were less than 2%(n=5)the average recovery was between 98.0%-101.0%.Conclusion:This method is simpleaccuratesensitive and applicable for determination of content and entrapment efficiency of lomustine liposomes.

PLEASE NOTE: The full text of this publication is in Chinese.

Keywords: RP-HPLCs; determination; entrapment efficiency; lomustine liposomes

Document Type: Research Article

Publication date: 01 March 2005

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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