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Separation of Monosialogangliosides(GM1) byCapillary Zone Electrophoresis

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To establish a method using capillary zone electrophoresis for separating two forms of monosialogangliosides(GM1).Method:The separation was performed on a fused-silica capillary 90 cm×75 μm(efficency length 83 cm).100 mmol·L-1 borax buffer containing 15 mmol·L-1-cyclodextrin(pH 9.9)was used as running buffer.The separation voltage was 30 kV.The UV detection wavelength was 200 nm.Results:The two forms of GM1 were successfully separated.Conclusion:This method can be applied to the seperation and the assay of two forms of GM1.

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Keywords: capillary zone electrophoresis; monosialogangliosides(GM1); α-cyclodextrin

Document Type: Research Article

Publication date: 01 March 2005

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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