Determination of Telmisartan in Human Plasma by HPLC with Fluorimetric Detection
To determine the concentration of telmisartan in human plasma by HPLC with fluorimetric detection.Method:Using naproxen as internal standard,telmisartan in plasma samples was determined by HPLC with liquid-liquid extraction of the compound from acidified plasma into organic solvent,achieved
by the column of Kromasil C8(150 mm×4.6 mm,5 μm)at room temperature.The mobile phase consisted of a mixture acetonitrile-0.02 mol·L-1potassium dihydrogen phosphate solution(50:50)pumped at a flow rate of 1.2 mL·min-1;A fluorescence detector was set at λEx
305 nm,λEm 365 nm.Results:The drug-free plasma did not interfere with the determination of drugs and internal standard.There was good linear relationships(1/C2 weighted)between peak area ratio of telmisartan to internal standard and C(r=0.999 6)within the range of 1-300 ng·mL-1.The
precision of within-day and between-day was good.The lower limit of quantification was 1 ng·mL-1.The analytes reconstituted in the mobile phase were also stable at ambient conditions(no control of temperature in the autosampler)for at least 24 h.Furthermore,telmisartan was stable for
at least three freeze thaw cycles.Conclusions:The method established in the paper can be used to determine the concentrations of telmisartan in human plasma.The method can also be applied to the clinical pharmaceutical research in vivo.
PLEASE NOTE: The full text of this publication is in Chinese.
Keywords: HPLC fluorescence; telmisartan
Document Type: Research Article
Publication date: 01 January 2005
- Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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