RP-HPLC Determination of 4 Active Components in Ramulus cinnamomi
A RP-HPLC method was established to determine the contents of cinnamaldehyde,cinnamic acid,coumarin and cinnamic alcohol in Ramulus cinnamomi.Method:The samples were separated on a Luna C18 column(4.6 mm×250 mm,5 μm)with the mobile phase of acetonitrile-0.1% phosphoric acid(28:72).The
flow rate was 1.0 mL·min-1,and the detection wavelength was set at 285 nm.Results:The linear ranges of cinnamaldehyde,cinnamic acid and cinnamic alcohol and coumarin were 0.2083.33 μg(r=0.9997),0.1001.60 μg(r=0.9999),0.2043.26 μg(r=0.9999),0.06741.08 μg(r=0.9999)respectively.The
average recoveries were 98.3%(RSD=1.0%),98.0%(RSD=1.4%),99.1%(RSD=1.2%),98.3%(RSD=1.8%)respectively.Conclusion:The method is simple and quick with good reproducibility,it is suitable for the determination of the contents of 4 active components.There is a correlation between contents of 4 active
components and the living environment of Ramulus cinnamomi and the collected season.
PLEASE NOTE: The full text of this publication is in Chinese.
Keywords: RP-HPLC; cinnamaldehyde; cinnamic acid; cinnamic alcohol; coumarin
Document Type: Research Article
Publication date: 01 March 2004
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