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RP-HPLC Determination of 4 Active Components in Ramulus cinnamomi

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A RP-HPLC method was established to determine the contents of cinnamaldehyde,cinnamic acid,coumarin and cinnamic alcohol in Ramulus cinnamomi.Method:The samples were separated on a Luna C18 column(4.6 mm×250 mm,5 μm)with the mobile phase of acetonitrile-0.1% phosphoric acid(28:72).The flow rate was 1.0 mL·min-1,and the detection wavelength was set at 285 nm.Results:The linear ranges of cinnamaldehyde,cinnamic acid and cinnamic alcohol and coumarin were 0.2083.33 μg(r=0.9997),0.1001.60 μg(r=0.9999),0.2043.26 μg(r=0.9999),0.06741.08 μg(r=0.9999)respectively.The average recoveries were 98.3%(RSD=1.0%),98.0%(RSD=1.4%),99.1%(RSD=1.2%),98.3%(RSD=1.8%)respectively.Conclusion:The method is simple and quick with good reproducibility,it is suitable for the determination of the contents of 4 active components.There is a correlation between contents of 4 active components and the living environment of Ramulus cinnamomi and the collected season.

PLEASE NOTE: The full text of this publication is in Chinese.

Keywords: RP-HPLC; cinnamaldehyde; cinnamic acid; cinnamic alcohol; coumarin

Document Type: Research Article

Publication date: 01 March 2004

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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