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Determination of Intraoellular Daunomycin in Leukemia Cells

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Assays with respectro fluorimetry and high performance liquid chromatography for determination of intracellular concentration of daunomycin in leukemia cells in vitro and iU rive have been establishedIt offers a method to study the accumulationmetabolism and intracellular pharmacokinetics of daunomycin in leukemia cellsThe fluorimetric method is applied to measurement of intracellular cOncentration of daunomycin in vitro because little metabolite Was found under that conditionHPLC method can be usEd either in vitro or in riveIn fluorimetric method 0.5ml 30trichloroa-cetic acid was added to 1.0ml cellulat lysateThe fluorescence of supernant was measured at excitation wavelength 475 nm and emission wavelength 590nmFor the in rive samples intracellular daunomycin was extracted by chloroform:methanol(4:1)Then the organic phase was evaporated under reduced pressureThe residue was dissolved in mobile phase and injected to HPLC(μ Bondapak C18 columnmobile phasel5mmol/L orthophosphoric acid:methanol:aeetonitri'e:iso-propanol(45:35:10:10)fluorescence deteeter and adriamycin as intemal standard)These two methods are highly sensitive with limit of detection of 2ng/ml and 5ng re spectivelyThe recovery is about 97

PLEASE NOTE: The full text of this publication is in Chinese.

Keywords: HPLC; intracellulaf coneentration of daunomycint fluoremetric methOd; leukemia cell

Document Type: Research Article

Publication date: 01 January 1992

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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