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ChomicaI Studies on the Alkaloids from Yuanhu(Corydalis fiurtschaninovii BessFYanhusuo YHChou et CCHsü)VHPLC Assay for Determination of Quate rnary Alkaloid s in Yuanhu

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A HPLC method was described for the determination of 4 quaternary alkaloids eolumbamine (A) coptislne (B)palmatine © and ehydrocorydaline (D)in the tuber of Corydalis turtschaninovii BessF.yanhvsvo YHChou et CCHasThe dried powdered sample was refluded with 20 ml MeOH for l hour The extract was adjusted into a 2ml MeOH solution of sampleand 35 μl are injected into a system comprising a column(2.6ram×25cm)of YWG 10 C18 equipped with a YWG 10 C18 guard columnwith CH3CN-0.04 M H3PO4(32:68)as mobile phase at 1.2ml/min and with detection at 349 nmThe separated components were determined by exteI'llal standards OU the basis of area valuesRectilinear responses were obtained in the range of 0.346.7μg with CV of 0.691.46Recoveries of the compounds added to the powdered sample were 99.4100.6 and 96.5% for columbaminepalmatine and dehydrocorydaline respectivelyThe results for different samples wore given

PLEASE NOTE: The full text of this publication is in Chinese.

Keywords: Columbamine; Coptisine; Dehydrocorydaline; HPLC; Palmatine; Quaternary alkaloids; Yuanhu(Corydalis turtschaminovii BessFyunhusuo YHCheu et et CCHas)

Document Type: Research Article

Publication date: 01 July 1986

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