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EXTRACTION AND DETERMINATION OF MICRO AMOUNT OF KETOMINE IN ANIMAL TISSUES

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Ketamine is a new kind of narcotic with rapid and short curative effectA simple method fof extraetion and determintion of micro amount of Ketamine inanimal tissues Was developedThe effects of the following factors on extraction were investigatedpH valuesdifferent solvents and methods of precipitation of proteinsThe proposed optimum method of extraction involves precipiation of proteins by trichloro-acetic acidAdjust of the sample solutions to pH>10 and extraction into etherGiving average recove ry of above 90Qualitativ e and quantitative analysis of the extracts by TLC (thin-layer chromatography ) and UV spectrophotometry were investigatedSeyen developing solvents and color reagent(bismuth potassium iodide)have been screened outKetamine shows the characteristic UV spectrum over the range of 200—300 nm in 0.1N H2SO4It can be determined spectrophotometriclly at 269 nm within the range from 0 to 400μg/mlThe TLC method is more sensitive than the UVand it is found to be more suitable for various cases

PLEASE NOTE: The full text of this publication is in Chinese.

Document Type: Research Article

Publication date: 01 January 1981

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  • Journal of Pharmaceutical Analysis is a professional journal administrated by China Association for Science and Technology, sponsored by Chinese Pharmaceutical Association, held by National Institutes for Food and Drug Control, edited and published by the Editorial Board of Journal of Pharmaceutical Analysis, and publicly distributed at home and abroad. It is one of the first group of journals that created by academic leaders in the field of drug control. It originated from Drug Control Communication which magazine was founded in 1951 and later been renamed in 1981. In 2005, it was changed from bi-monthly journal to monthly journal. Journal of Pharmaceutical Analysis is among core journals in nature sciences and even other Chinese core journals, whose main columns are research reports, communications, review, forum, information and etc. It mainly reports the latest research results, explore new theories, introduce new development, spread new techniques, and promote new methods of pharmaceutical analysis. Its contents include peer-reviewed research reports, research presentation and summary reviews on all aspects of pharmaceutical analysis including drug development, pharmaceutical manufacturing, clinical research, drug safety, quality assessment, and market surveillance. Main authors and target reader are professional and technical personnel engaged in research, application as well as higher education in pharmaceutical analysis.
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